Journal
FOOD CHEMISTRY
Volume 246, Issue -, Pages 437-441Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2017.12.022
Keywords
Paper-based microfluidics; Food safety; Clenbuterol; Milk; ELISA; Immunoassay
Funding
- Natural Sciences and Engineering Research Council of Canada [NSERC CRDPJ 486586-15]
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In this study, a paper-based microfluidic enzyme-linked immunosorbent assay (ELISA) was developed as a screening system for rapid detection of clenbuterol, which is illegally used as a growth promoter for food-producing animals. The microfluidic paper-based analytical device (mu PAD) was combined with ELISA and the intrinsic properties of paper allowed the entrapment of antibody through cellulosic fibres, validating to be an alternative to 96-well ELISA microplate for food safety monitoring. Detection of clenbuterol in milk was achieved by measuring the intensity of colour change that was proportional to the analyte concentration with a detection limit of 0.2 ppb. The mu PAD effectively reduces the cost, volume of reagents, and time required to run ELISA for food sample testing.
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