Journal
FOOD CHEMISTRY
Volume 250, Issue -, Pages 134-139Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2017.12.079
Keywords
Molecular imprinting; Hydrophilic imprinted film; Biomimetic immunosorbent assay; Quantum dots
Funding
- National Natural Science Foundation of China [31471649]
- National Key Research and Development Program of China [2016YFD0401201-7]
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A direct competitive biomimetic immunosorbent assay method based on molecularly imprinted polymer was developed for the determination of trichlorfon. A CdSe/ZnS quantum dot label was used as the marker. The hydrophilic imprinted film was synthesized directly on the surface of a 96-well plate, and characterized by Fourier-transform infrared spectroscopy and thermo-gravimetric analyses. The method exhibited high stability, selectivity, and sensitivity. Under optimal conditions, the limits of detection and sensitivity of the biomimetic immunosorbent assay method were 9.0 mu g L-1 and 5.0 mg L-1 (0.1 mg kg(-1) and 62.5 mg kg(-1) for vegetable sample), respectively. Low cross-reactivity values of 19.2% and 15.6% were obtained for the structural analogues. Spinach and rape samples spiked with trichlorfon were extracted and determined by this method with recoveries ranging from 83.6% to 91.1%. The method was applied for the detection of trichlorfon residues in leek and cucumber samples, and results correlated well with those obtained using GC.
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