Journal
FOOD CHEMISTRY
Volume 249, Issue -, Pages 136-142Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2018.01.025
Keywords
UCNPs; FRET; PAT; Gold nanoparticles; Aptamer
Funding
- Agricultural Research Service project [1935-42000-049-00D]
- Center for Food Safety Engineering at Purdue University
- Nature Science Foundation of China [31601413, 31501418]
- graduate student innovation project of Jiangsu province [KYLX15_1134]
- National Key Technology R&D Program for the 12th Five-Year Plan [2016YFD0400304]
- independent research project program of the State Key Laboratory of Food Science and Technology of Jiangnan University [JUSRP1029]
- Chinese Scholarship Council (CSC)
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Patulin (PAT) is a type of mycotoxin which can compromise both food quality and human health. Due to its harmful effects, strict monitoring of PAT contents in food systems is necessary. A novel kind of bioassay was proposed for determining PAT based on the fluorescent resonant energy transfer (FRET) strategy. The exonuclease-catalyzed target recycling strategy was employed to improve the sensitivity of the FRET system. The results showed that the linear range extends from 0.01 ng/mL to 100 ng/mL. Furthermore, the average recoveries ranged from 93.33% to 105.21%, confirming the reliability of this method. The total analysis time for our assay developed is about 50 min. Compared to traditional analytical methods, the developed assay is more stable and has a significantly lower detection of limit (0.003 ng/mL). We believe the approach developed in this study would be useful for high-throughput screening of PAT in food industry and government laboratory.
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