Journal
FOOD CHEMISTRY
Volume 256, Issue -, Pages 91-97Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2018.02.118
Keywords
Bromelain; Surface imprinting; Magnetic mesoporous carbon; Biomacromolecular absorption
Funding
- National Natural Science Foundations of China [81573391, 81773689, 81503034, 81603073]
- China Postdoctoral Science Foundation [2016M602836, 2016M592774]
- Shaanxi Province Postdoctoral Science Foundation [2016BSHYDZZ13]
- Shaanxi Province Natural Science Foundation of China [2017JQ2027]
- Cross Research Funds of Xi'an Jiaotong University [xjj2017185]
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Bromelain, a cysteine endopeptidase enzyme of great commercial value, has been widely used in food, pharmaceutical, and cosmetic industries. Conventional methods for purification of bromelain are still limited by a low binding efficiency, time-consuming process, and expensive equipment. Therefore, for selective absorption of bromelain, we developed a facile and effective method to fabricate magnetic mesoporous molecularly imprinted polymers using pericarpium granati-derived carbon as the carrier for the first time. The characterizations of the imprinted polymers indicated that a polydopamine layer was coated on the surface of the carrier and the crystallinity of the carrier did not change. The obtained imprinted polymers exhibited favourable saturation magnetization, a high adsorption capacity of 135.96 mg g(-1), a fast equilibrium time, and satisfactory reusability. The imprinted polymers were prepared by an eco-friendly method and exhibited rapid separation and good adsorption performance, thus making the method applicable to biomacromolecular separation, proteomic analysis, and biomedical research.
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