Journal
BULLETIN OF THE VETERINARY INSTITUTE IN PULAWY
Volume 59, Issue 1, Pages 85-90Publisher
NATL VETERINARY RESEARCH INST
DOI: 10.1515/bvip-2015-0013
Keywords
dog; semen; lowered fertility; selenium; vitamin E
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Thirty clinically healthy dogs with poor semen qualik were used in the study. Fifteen dogs were supplemented daily with selenium ((1.6 mg/kg organic selenium from yeast) and vitamin E (5 mg/kg) per as for 60 d. the control group (15 dogs) was not supplemented. Semen was collected from all dogs by manual manipulation on days 0, 30, 60, and 90. the sperm concentration and motility parameters were evaluated with a Hamilton Thorne sperm analyser, version IVOS 12.3. For the assessment of sperm morpholog), Diff-Quik stain was used. The percentage of live and dead spermatozoa was estimated on dried smears stained with eosin-nigrosin. The concentration of spermatozoa, most motility parameters determined (PMOT, VSL, VCL, ALIL, BCF, MEDIUM, SLOW, and STATIC), and the percentage of spermatozoa morphologicaly normal and live increased significantly (P = 0.05) after 60 d of supplementation. In the control group, there were no changes in motility parameters while the concentration and total sperm count decreased over the duration of the study. In conclusion, supplementation with selenium and vitamin E for 60 d can improve the quality of semen in dogs with lowered fertility.
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