Journal
FOOD CHEMISTRY
Volume 268, Issue -, Pages 242-248Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2018.06.096
Keywords
Deoxynivalenol; Mimic epitope peptide; Enzyme-linked immunosorbent assay; Immunochromatographic assay
Funding
- National Natural Science Foundation of China, China [81660660, 31471648, 31671837]
- Project of the Goal-oriented
- Open Project Program of State Key Laboratory of Food Science and Technology, Nanchang University [SKLF-ZZA-201612, SKLF-KF-201610]
- Doctoral Scientific Fund Project of the Ministry of Education of China [20123601110004]
- [20172BCB22006]
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A deoxynivalenol (DON) epitope clone (D-8) was obtained by phage display technology using anti-DON monoclonal antibodies as a target molecule. Subsequently, a DON antigen mimic (D-8-maltose-binding protein [MBP]) was synthesized by fusing the mimic epitope peptide with MBP. An enzyme-linked immunosorbent assay (ELISA) and urchin-like gold nanoparticle immunochromatographic assay was developed based on D-8-MBP for detection of DON in maize and wheat. The half-maximal inhibitory concentration, lower detection limit, and linear range of the D-8-MBP ELISA were 57.98 +/- 0.97, 9.83, and 11.32-286.77 ng/mL, respectively. The sensitivity of the D-8-MBP ELISA was nearly 2.5 times higher than that of traditional ELISA using DON-bovine serum albumin (BSA). The detection threshold of the colloidal gold immunochromatographic assay for D-8-MBP was 25 ng/mL. Thus, D-8-MBP could be used to replace the traditional DON-BSA antigen for the immunological detection of DON, permitting low cost, rapid detection of DON.
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