Journal
FISH PHYSIOLOGY AND BIOCHEMISTRY
Volume 44, Issue 6, Pages 1435-1442Publisher
SPRINGER
DOI: 10.1007/s10695-018-0491-3
Keywords
Common carp; Sodium; Potassium; pH; Artificial seminal plasma; Sperm preservation; Chilled storage
Categories
Funding
- KNOW Consortium grant Healthy Animal - Safe Food [05-1/KNOW2/2015]
- European Fisheries Fund Fisheries Operative Programme III axis
- European Fisheries Fund for Renewable Fisheries
- EU [EFOP-3.6.3-VEKOP-16-2017-00008]
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The effect of sodium and potassium concentrations as well as optimal pH on the motility of common carp Cyprinus carpio L. sperm during short-term storage in artificial seminal plasma (ASP) was investigated. Sperm was collected from individual males (n=5) and each sample diluted tenfold (1:9) in ASP (sperm:extender) containing 2mM CaCl2, 1mM Mg2SO4 and 20mM Tris at pH8.0 and supplemented by the following concentrations of sodium and potassium (mM/mM): 0/150, 20/130, 40/110, 75/75, 110/40, 130/20 and 150/0. The osmolality of all ASP variants was set at 310mOsmkg(-1). Sperm motility was measured using a CASA system during 72h of storage. Immediately after dilution, sperm motility was high (90%) both in each variant and in the control group (fresh sperm). After 72-h storage, the highest sperm motility was noted in ASP containing 110mM NaCl and 40mM KCl. No differences were found in the motility of samples preserved within the pH range of 7.0-9.0. Our data suggest that for the short-term storage of common carp sperm, whereas the pH of the solution does not play a crucial role, a specific potassium concentration of around 40mM is required.
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