The Use of 31P NMR for Quantitative Analysis of Distarch Phosphate and Determination of the Reaction Mechanism

Distarch phosphates of different degree of substitution were analysed by P-31 NMR. A major part of P was inorganic. When analysing reaction products of wheat starch in laboratory, cyclic monophosphate as a reaction intermediate was found. The chosen procedure made it possible to reach the DS 0.002.