Journal
FEBS LETTERS
Volume 592, Issue 8, Pages 1412-1425Publisher
WILEY
DOI: 10.1002/1873-3468.13032
Keywords
Enterococcus faecalis; gene expression; global regulators; protein-DNA interactions
Funding
- AEI/FEDER, UE [BIO2016-76412-C2-2-R]
- Spanish Ministry of Economy and Competitiveness [BIO2015-69085-REDC]
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Global transcriptional regulators play key roles during bacterial adaptation to environmental fluctuations. Protein MafR from Enterococcus faecalis was shown to activate the transcription of many genes on a genome-wide scale. We proposed that MafR is a global regulator of the Mga/AtxA family. Here, we purified an untagged form of the MafR protein and found that it binds to linear double-stranded DNAs in a nonsequence-specific manner. Moreover, multiple MafR units (likely dieters) bind sequentially to the DNA molecule generating multimeric complexes. On DNAs that contain the promoter of the mafR gene, MafR recognizes a potentially curved DNA region. We discuss that a characteristic of the Mga/AtxA regulators might be their ability to recognize particular DNA shapes across the bacterial genomes.
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