Journal
FASEB JOURNAL
Volume 32, Issue 8, Pages 4600-4611Publisher
FEDERATION AMER SOC EXP BIOL
DOI: 10.1096/fj.201701462RR
Keywords
surface tension; purinergic signaling; P2Y(2); calcium; lung surfactant
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Funding
- Spanish Ministry of Economy and Competitiveness [BIO2012-30733, BIO2015-67930-R, EEBB-I-2015-10363]
- Regional Government of Madrid [P2013/MIT-2807]
- Deutsche Forschungsgemeinschaft (DFG) [DI1402/3-1]
- Ministry of Science, Research, and the Arts of Baden-Wurttemberg [Az: 32-7533.-6-10/15/5]
- DFG [SFB 1149/1, A05]
- Formacion de Personal Investigador (FPI) Fellowship from the Spanish Ministry of Economy and Competitiveness
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The release of pulmonary surfactant by alveolar type II (ATII) cells is essential for lowering surface tension at the respiratory air-liquid interface, stabilizing the lungs against physical forces tending to alveolar collapse. Hydrophobic surfactant protein (SP)-B ensures the proper packing of newly synthesized surfactant particles, promotes the formation of the surface active film at the alveolar air-liquid interface and maintains its proper structure along the respiratory dynamics. We report that membrane-associated SP-B efficiently induces secretion of pulmonary surfactant by ATII cells, at the same level as potent secretagogues such as ATP. The presence in the extracellular medium of lipid-protein complexes containing SP-B activates the P2Y(2) purinergic signaling pathway that ultimately triggers exocytosis of lamellar bodies by ATII cells. Our data suggest that SP-B prompts Ca2+-dependent surfactant secretion via ATP release from ATII cells. This result implies that SP-B is not only an essential component for the biophysical function of surfactant but is also a central element in the alveolar homeostasis by eliciting autocrine and paracrine cell stimulation.Martinez-Calle, M., Olmeda, B., Dietl, P., Frick, M., Perez-Gil, J. Pulmonary surfactant protein SP-B promotes exocytosis of lamellar bodies in alveolar type II cells.
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