Journal
MOLECULAR BIOTECHNOLOGY
Volume 57, Issue 6, Pages 558-564Publisher
HUMANA PRESS INC
DOI: 10.1007/s12033-015-9849-2
Keywords
Antimicrobial resistance; Microbial biosensor; Pseudomonas; Extrusion efflux pump; TtgR repressor
Funding
- Spanish Ministry of Economy and Competitiveness [RYC-2009-04570]
- Junta de Andalucia [P11-CVI-7391]
- EFDR
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Environmental contamination by toxic organic compounds and antimicrobials is one of the causes for the recent surge of multidrug-resistant pathogenic bacteria. Monitoring contamination is therefore the first step in containment of antimicrobial resistance and requires the development of simple, sensitive, and quantitative tools that detect a broad spectrum of toxic compounds. In this study, we have engineered a new microbial biosensor based on the ttgR-regulated promoter that controls expression of the TtgABC extrusion efflux pump of Pseudomonas putida, coupled to a gfp reporter. The system was introduced in P. putida DOT-T1E, a strain characterized by its ability to survive in the presence of high concentrations of diverse toxic organic compounds. This whole-cell biosensor is capable to detect a wide range of structurally diverse antibiotics, as well as compounds such as toluene or flavonoids.
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