Journal
FUNGAL GENETICS AND BIOLOGY
Volume 79, Issue -, Pages 84-88Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.fgb.2015.04.006
Keywords
RNAi; Mycosphaerella graminicola; Septoria tritici; Wheat; Triticum aestivum; Virus induced gene silencing (VIGS); Functional genomics
Categories
Funding
- Biotechnology and Biological Sciences Research Council of the UK (BBSRC) [BB/J/00426X/1]
- Biotechnology and Biological Sciences Research Council [BBS/E/C/00005203, BB/I025077/1] Funding Source: researchfish
- BBSRC [BB/I025077/1, BBS/E/C/00005203] Funding Source: UKRI
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Virus-induced gene silencing (VIGS) has emerged as a powerful reverse genetic technology in plants supplementary to stable transgenic RNAi and, in certain species, as a viable alternative approach for gene functional analysis. The RNA virus Barley stripe mosaic virus (BSMV) was developed as a VIGS vector in the early 2000s and since then it has been used to study the function of wheat genes. Several variants of BSMV vectors are available, with some requiring in vitro transcription of infectious viral RNA, while others rely on in planta production of viral RNA from DNA-based vectors delivered to plant cells either by particle bombardment or Agrobacterium tumefaciens. We adapted the latest generation of binary BSMV VIGS vectors for the identification and study of wheat genes of interest involved in interactions with Zymoseptoria tritici and here present detailed and the most up-to-date protocols. (C) 2015 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
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