Journal
EXPERIMENTAL PHYSIOLOGY
Volume 103, Issue 6, Pages 916-923Publisher
WILEY
DOI: 10.1113/EP086957
Keywords
angiotensin-(1-7); angiotensin-converting enzyme 2; cerebral blood flow; ischaemic stroke; neuroprotection
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Funding
- National Heart Lung and Blood Institute [2T32HL083810-06A1]
- American Heart Association [12PRE11940010]
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [T32HL083810] Funding Source: NIH RePORTER
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As a target for stroke therapies, the angiotensin-converting enzyme 2-angiotensin-(1-7)-Mas [ACE2/Ang-(1-7)/Mas] axis of the renin-angiotensin system can be activated chronically to induce neuroprotective effects, in opposition to the deleterious effects of angiotensinII via its type1 receptor. However, more clinically relevant treatment protocols with Ang-(1-7) that involve its systemic administration beginning after the onset of ischaemia have not been tested. In this study, we tested systemic post-stroke treatments using a molecule where Ang-(1-7) is included within hydroxypropyl--cyclodextrin [HPCD-Ang-(1-7)] as an orally bioavailable treatment. In three separate protocols, HPCD-Ang-(1-7) was administered orally to Sprague-Dawley rats after induction of ischaemic stroke by endothelin-1-induced middle cerebral artery occlusion: (i) to assess its effects on cerebral damage and behavioural deficits; (ii) to determine its effects on cardiovascular parameters; and (iii) to determine whether it altered cerebral blood flow. The results indicate that post-stroke oral administration of HPCD-Ang-(1-7) resulted in 25% reductions in cerebral infarct volumes and improvement in neurological functions (P<0.05), without inducing any alterations in blood pressure, heart rate or cerebral blood flow. In conclusion, Ang-(1-7) treatment using an oral formulation after the onset of ischaemia induces significant neuroprotection in stroke and might represent a viable approach for taking advantage of the protective ACE2/Ang-(1-7)/Mas axis in this disease.
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