Journal
FUNGAL BIOLOGY
Volume 119, Issue 11, Pages 973-983Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.funbio.2015.06.006
Keywords
Secondary metabolite
Categories
Funding
- National Research Foundation of Korea [NRF 2010-0012426]
- Agriculture, Food and Rural Affairs Research Center Support Program, Ministry of Agriculture, Food and Rural Affairs
- World-Class 300 project, Small and Medium Business Administration of the Republic of Korea
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To explore novel bioactive compounds produced via activation of secondary metabolite (SM) gene clusters, we overexpressed an ortholog of laeA, a gene that encodes a global positive regulator of secondary metabolism in Aspergillus fumisynnematus F746. Overexpression of the laeA gene under the alcA promoter resulted in the production of less pigment, shorter conidial head chains, and fewer conidia. Thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC) analysis revealed that SM production in OE::laeA was significantly increased, and included new metabolites that were not detected in the wild type. Among them, a compound named F1 was selected on the basis of its high production levels and antibacterial effects. F1 was purified by column chromatography and preparative TLC and identified as cyclopiazonic acid (CPA) by LC/MS, which had been previously known as mycotoxin. As A. fumisynnematus was not known to produce CPA, these results suggest that overexpression of the laeA gene can be used to explore the synthesis of useful bioactive compounds, even in a fungus for which the genome sequence is unavailable. (C) 2015 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.
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