Journal
EXPERIMENTAL CELL RESEARCH
Volume 366, Issue 1, Pages 49-54Publisher
ELSEVIER INC
DOI: 10.1016/j.yexcr.2018.03.011
Keywords
GLUT4; Insulin; SLC2A4; Cadherin; Actin cytoskeleton; Wnt; beta-catenin
Categories
Funding
- Health Research Council of New Zealand
- University of Auckland Doctoral Scholarship
- Maurice Wilkins Centre Travelling Fellowship
- Rutherford Post Doctoral Research Fellowship from the Royal Society of New Zealand
Ask authors/readers for more resources
GLUT4 is unique among specialized glucose transporters in being exclusively expressed in muscle and adipocytes. In the absence of insulin the distribution of GLUT4 is preferentially intracellular and insulin stimulation results in the movement of GLUT4 containing vesicles to the plasma membrane. This process is responsible for the insulin stimulation of glucose uptake in muscle and fat. While signalling pathways triggering the translocation of GLUT4 are well understood, the mechanisms regulating the intracellular retention of GLUT4 are less well understood. Here we report a role for beta-catenin in this process. In 3T3-L1 adipocytes in which beta-catenin is depleted, the levels of GLUT4 at and near the plasma membrane rise in unstimulated cells while the subsequent increase in GLUT4 at the plasma membrane upon insulin stimulation is reduced. Small molecule approaches to acutely activate or inhibit beta-catenin give results that support the results obtained with siRNA and these changes are accompanied by matching changes in glucose transport into these cells. Together these results indicate that beta-catenin is a previously unrecognized regulator of the mechanisms that control the insulin sensitive pool of GLUT4 transporters inside these adipocyte cells.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available