Journal
RSC ADVANCES
Volume 5, Issue 54, Pages 43480-43488Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5ra04308e
Keywords
-
Categories
Funding
- Saxon Ministry for Higher Education, Research and Arts (SMWK) [4-7531.60/29/24]
- Shanghai Municipal NaturalScience Foundation [14ZR1445500]
- Natural Science Foundation of China [31400807]
Ask authors/readers for more resources
In the present work, gelatin/alginate and gelatin/alginate/hydroxyapatite (HAP) composite scaffolds were fabricated by 3D plotting based on high concentration gelatin/alginate pastes. At temperatures of 37 degrees C or above, the developed pastes could be easily processed into designed 3D structures; sequential crosslinking with Ca2+ ions (effective for alginate) and the carbodiimide EDC (effective for gelatin) resulted in stable scaffolds. Mechanical testing demonstrated that the plotted composite scaffolds had a significantly higher strength and modulus compared to most reported gelatin scaffolds prepared by conventional methods. Cell experiments with human bone marrow-derived mesenchymal stem cells (hBMSC) revealed that the gelatin/alginate composite scaffolds favor cell adhesion and support proliferation. Furthermore, the cells showed a homogeneous distribution and excellent migration in the inner regions of the plotted composite scaffolds over 21 days. In conclusion, gelatin/alginate scaffolds, with or without HAP, fabricated by 3D plotting according to a predesigned CAD-model might be potential candidates for the repair of bony and chondral defects, especially in complex defect situations affecting the osteochondral tissue interface since biphasic scaffolds with a stable connection of the two parts can be easily fabricated by multi-channel 3D plotting.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available