Regulatory Tcells are required for normal and activin-promoted wound repair in mice

Healing of skin wounds is orchestrated by various types of immune cells, but little is known about the role of FoxP3(+) regulatory Tcells (Tregs) in this process. Here, we determined if Tregs are important for wound healing in normal mice and if they contribute to the accelerated healing of mice overexpressing the growth and differentiation factor activin. Diphtheria toxin induced Treg depletion prior to injury caused impaired healing characterized by delayed reepithelialization, reduced wound contraction, and impaired vessel maturation. The accelerated wound repair of activin-transgenic mice was also abrogated. Mechanistically, we found a strong increase in IL-4 levels combined with overrepresentation of T-bet(+) and GATA-3(+) Tcells in Treg-depleted 7-day wounds. In addition, numbers of IFN-- or IL-17A-producing CD4(+) and CD4(-) Tcells were elevated. These results demonstrate that Treg depletion in wounds facilitates the expansion of an T-cell population with features of Th1 and Th2 cells, and suggest that concomitant changes in the cytokine milieu disturb the healing process.