Journal
EUROPEAN JOURNAL OF IMMUNOLOGY
Volume 48, Issue 5, Pages 855-860Publisher
WILEY
DOI: 10.1002/eji.201747283
Keywords
Axl; IFN-alpha; MerTK; Monocyte-derived macrophages; TAM receptor
Categories
Funding
- National Science Centre, Poland [UMO-2015/19/P/NZ7/03659]
- European Union [665778]
- European Society of Paediatric Infectious Diseases
- Medical Research Council [MR/N001427/1]
- Wellcome Trust [202865/Z/16/Z]
- GlaxoSmithKline
- AstraZeneca
- MRC [G0802752, MC_PC_15072, MR/N001427/1] Funding Source: UKRI
- Wellcome Trust [202865/Z/16/Z] Funding Source: Wellcome Trust
- Medical Research Council [MR/N001427/1, G0802752, MC_PC_15072] Funding Source: researchfish
- Wellcome Trust [202865/Z/16/Z] Funding Source: researchfish
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The requirement to remove apoptotic cells is equally important in homeostasis and inflammatory disease. In particular, during viral infections large quantities of infected cells undergo apoptosis and need to be efficiently cleared by phagocytes to prevent secondary necrosis. Although specific roles of several apoptotic cell sensors, such as the TAM (Tyro3, Axl, MerTK) receptor family, have been characterized in mouse models, little is known about their regulation and involvement in apoptotic cell uptake (efferocytosis) by human macrophages under inflammatory conditions. We show that whereas pro-inflammatory stimuli consistently downregulated MerTK expression in human monocyte-derived macrophages (MDMs), stimuli indicative of a viral infection, interferon-alpha (IFN-alpha) and the TLR3 ligand poly(I:C), specifically induced Axl expression and promoted binding of the bridging molecule Gas6. Axl induction by IFN-alpha and poly(I:C) was associated with higher MDM efferocytic capacity compared to cells treated with other pro-inflammatory stimuli, such as LPS and IFN-gamma. While MerTK blocking antibody uniformly suppressed apoptotic cell uptake by MDMs, Axl blocking antibody significantly reduced efferocytosis by poly(I:C)-stimulated MDMs, but not by resting MDMs. Our observations demonstrate that Axl induction during viral infections contributes to maintaining macrophage capacity to engulf apoptotic cells, which may have important consequences for resolution of anti-viral immune responses.
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