Journal
NATURE PLANTS
Volume 1, Issue 7, Pages -Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NPLANTS.2015.94
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Funding
- Marie Curie Initial Training Network 'BRAVISSIMO' [PITN-GA-2008-215118]
- Odysseus program of the Research Foundation-Flanders
- Max-Planck Gesellschaft
- European Union Seventh Framework Programme (FP7) (WallTrac, Marie Curie Initial Training Network) [263916]
- Deutsche Forschungsgemeinschaft [SFB1101, TPA02]
- Belgian Science Policy Office (BELSPO)
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In plants, vacuolar H+-ATPase (V-ATPase) activity acidifies both the trans-Golgi network/early endosome (TGN/EE) and the vacuole. This dual V-ATPase function has impeded our understanding of how the pH homeostasis within the plant TGN/EE controls exo- and endocytosis. Here, we show that the weak V-ATPase mutant deetiolated3 (det3) displayed a pH increase in the TGN/EE, but not in the vacuole, strongly impairing secretion and recycling of the brassinosteroid receptor and the cellulose synthase complexes to the plasma membrane, in contrast to mutants lacking tonoplast-localized V-ATPase activity only. The brassinosteroid insensitivity and the cellulose deficiency defects in det3 were tightly correlated with reduced Golgi and TGN/EE motility. Thus, our results provide strong evidence that acidification of the TGN/EE, but not of the vacuole, is indispensable for functional secretion and recycling in plants.
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