Journal
FUEL
Volume 140, Issue -, Pages 717-723Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.fuel.2014.10.019
Keywords
HPLC; Reverse phase; High resolution mass spectrometry; APPI; Petroleomics
Categories
Funding
- Korea Research Institute of Standards and Science [14011049]
- Ministry of Land, Transport and Maritime Affairs, ROK [PM56951]
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A reversed-phase separation technique was developed in a previous study (Loegel et al., 2012) and successfully applied to the de-asphalted fraction of crude oil. However, to the best of our knowledge, the molecular-level characterization of oil fractions obtained by reversed-phase high-performance liquid chromatography (HPLC) coupled with high-resolution mass spectrometry (MS) has not yet been reported. A detailed characterization of the oil fractions prepared by reversed-phase HPLC was performed in this study. HPLC fractionation was carried out on conventional crude oil and an oil shale pyrolysate. The analyses of the fractions showed that the carbon number of alkyl chains and the double bond equivalent (DBE) value were the major factors determining elution order. The compounds with larger DBE (presumably more condensed aromatic structures) and smaller carbon number (presumably compounds with short side chains) were eluted earlier but those compounds with lower DBE values (presumably less aromatic structures) and higher carbon number (presumably compounds with longer alkyl chains) eluted later in the chromatograms. This separation behavior is in good agreement with that expected from the principles of reversed-phase separation. The data presented in this study show that reversed-phase chromatography is effective in separating crude oil compounds and can be combined with ultrahighresolution MS data to better understand natural oils and oil shale pyrolysates. (C) 2014 Elsevier Ltd. All rights reserved.
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