Journal
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
Volume 25, Issue 15, Pages 14544-14550Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s11356-018-1686-y
Keywords
Cd2+; Phycocyanin; Toxicity; Fluorescence characteristics; Molecule structure
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Funding
- National Natural Science Foundation of China [21707026]
- Guangzhou Key Laboratory of Environmental Exposure and Health [GZKLEEH201613]
- Shandong Provincial Natural Science Foundation, China [ZR2014BQ033]
- Natural Scientific Research Innovation Foundation in Harbin Institute of Technology [HIT.NSRIF.2014126]
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The pollution of heavy metals is a severer problem for the ecosystems in waters. The toxicity of Cd2+ on phycocyanin (PC) is studied in molecular level in this work. The fluorescence quenching of PC is observed by the adding Cd2+ from 0 to 500 x 10(-7) mol L-1. From the theoretical calculation and the time-resolved fluorescence decay profiles, the fluorescence quenching of PC by Cd2+ is found to be static. The synchronous fluorescence spectra are used to study the change in amino acid residues of PC molecules, indicating that the effect of Cd2+ on the Trp of PC is more significant than the Tyr. The UV-Vis absorbance of tetrapyrrole decreases from 0.26 to 0.23 cps with increasing Cd2+ concentration, suggesting that Cd2+ affects the light adsorption and the photosynthesis function of PC. The circular dichroism spectra reveal that adding Cd2+ also changes the secondary structure (alpha-helix) of PC.
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