Journal
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
Volume 155, Issue -, Pages 142-157Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.envexpbot.2018.06.031
Keywords
Heat shock proteins (HSPs); Salinity stress; Virus induced gene silencing (VIGS); Nicotianabenthamiana; PCA (principle component analysis); HCA (hierarchical clustering analysis); Growth physiological parameters
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Funding
- University of Isfahan
- University of Kashan [Uki0015]
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Heat shock proteins (HSPs) have vital roles during plant adaptation to biotic and abiotic stresses, as well as stress-free conditions. In the present study, we used a heterologous strategy of virus induced gene silencing to investigate the role of different classes of HSPs in Nicotiana benthamiana. Different growth and physiological parameters in silenced plants were evaluated under both control and salt stress conditions. Among the treatments, silencing of HSP70, especially under salinity regime, was found to have stronger impacts on growth, protein concentration, the accumulation of photosynthetic pigments, proline and total soluble carbohydrates content, malondialdehyde, the activity of antioxidant enzymes, performance index, relative water content and the ratio of K+/Na+, suggesting a more prominent role for HSP70 in both stressful and stress-free life cycle of the plants. Principal component analysis and hierarchical clustering indicated that HSP70 gene was silenced, the plants might effectively respond to stress by a higher accumulation of compatible solutes, like proline to protect the cell membranes and proteins from damage.
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