Journal
NEPHRON
Volume 130, Issue 3, Pages 191-199Publisher
KARGER
DOI: 10.1159/000433566
Keywords
Organoids; Dissociation reaggregation systems; Pluripotent stem cells; Three-dimensional cultures; Modelling of development and disease; Regenerative medicine
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Funding
- Associazione per la Ricerca sul Diabete - Italia
- Bellco Srl
- [ERC-2010-AdG-268632]
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Recent technical advances in the stem cell field have enabled the in vitro generation of complex structures resembling whole organs termed organoids. Most of these approaches employ three-dimensional (3D) culture systems that allow stem cell-derived or tissue progenitor cells to self-organize into 3D structures. These systems evolved, methodologically and conceptually, from classical reaggregation experiments, showing that dissociated cells from embryonic organs can reaggregate and re-create the original organ architecture. Since organoids can be grown from human stem cells and from patient-derived induced pluripotent stem cells, they create significant prospects for modelling development and diseases, for toxicology and drug discovery studies, and in the field of regenerative medicine. Here, we outline historical advances in the field and describe some of the major recent developments in 3D human organoid formation. Finally, we underline current limitations and highlight examples of how organoid technology can be applied in biomedical research. (C) 2015 S. Karger AG, Basel
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