Journal
EMBO JOURNAL
Volume 37, Issue 4, Pages -Publisher
WILEY
DOI: 10.15252/embj.201797490
Keywords
pervasive transcription; Rap1; roadblock termination; transcription readthrough; transcription termination mechanism
Categories
Funding
- Centre National de la Recherche Scientifique (C.N.R.S.)
- Fondation pour la Recherche Medicale (F.R.M)
- l'Agence Nationale pour la Recherche (A.N.R.) [ANR-08-Blan-0038-01, ANR-12-BSV8-0014-01, ANR-16-CE12-0022-01]
- Fondation Bettencourt (prix Coup d'Elan)
- French Ministry of Research
- LABEX Who am I
- Agence Nationale de la Recherche (ANR) [ANR-16-CE12-0022, ANR-08-BLAN-0038, ANR-12-BSV8-0014] Funding Source: Agence Nationale de la Recherche (ANR)
Ask authors/readers for more resources
Transcription termination delimits transcription units but also plays important roles in limiting pervasive transcription. We have previously shown that transcription termination occurs when elongating RNA polymerase II (RNAPII) collides with the DNA-bound general transcription factor Reb1. We demonstrate here that many different DNA-binding proteins can induce termination by a similar roadblock (RB) mechanism. We generated high-resolution transcription maps by the direct detection of RNAPII upon nuclear depletion of two essential RB factors or when the canonical termination pathways for coding and non-coding RNAs are defective. We show that RB termination occurs genomewide and functions independently of (and redundantly with) the main transcription termination pathways. We provide evidence that transcriptional readthrough at canonical terminators is a significant source of pervasive transcription, which is controlled to a large extent by RB termination. Finally, we demonstrate the occurrence of RB termination around centro-meres and tRNA genes, which we suggest shields these regions from RNAPII to preserve their functional integrity.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available