Journal
ELECTROPHORESIS
Volume 39, Issue 16, Pages 2125-2130Publisher
WILEY
DOI: 10.1002/elps.201800055
Keywords
Cereals; Double-label immunochromatographic assay; Quantitative; Zearalenone
Funding
- National Program for support of Top-notch Young Professionals
- Special Fund for Grain-scientific Research in the Public Interest [201513006-02]
- Hubei Academy of Agricultural Sciences [2015jzxjh06]
- National Key Research and Development Program of China [2016YFF0201901]
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A double-label immunochromatographic based assay (DL-ICA) was developed to monitor zearalenone (ZEN) levels in cereals, based on Eu3+ nanoparticles (EuNP). The DL-ICA exhibited excellent sensitivity, reliability and selectivity in real samples. It showed low limits of detection (0.21-0.25 g/kg) and broad analytical ranges (up to 120 g/kg). The total analytical time, including sample preparation and DL-ICA execution, was reduced by 15 min compared with HPLC. The recovery rates ranged from 95.0-118.4%, with relative standard deviations (RSD) <11.6%. Inter- and intra-day validations were assessed, recovery rates of 89.3-106.9% and RSD of 2.3-9.7% were obtained, suggesting considerable stability and reliability for the assay. An excellent correlation was observed between DL-ICA and a reference HPLC method (R-2 = 0.9899). Compared to current immunoassays, the current DL-ICA is inexpensive, highly sensitive, and rapid. Therefore, DL-ICA constitutes a novel tool for monitoring mycotoxins in food and feed to ensure safety.
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