Journal
ELECTROPHORESIS
Volume 39, Issue 16, Pages 2117-2124Publisher
WILEY
DOI: 10.1002/elps.201800102
Keywords
Capillary electrophoresis; Flavonoid; Field amplified sample stacking; Goji berry
Funding
- National Natural Science Foundation of China [81673325, 81711540311, 21705156]
- Central Asia Drug Discovery and Development Centre of the Chinese Academy of Sciences [CAM 201704]
- CAS Pioneer Hundred Talents Program
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Goji berry, fruits of the plant Lycium barbarum L., has long been used as traditional medicine and functional food in China. In this work, a simple and easy-operation on-line concentration capillary electrophoresis (CE) for detection flavonoids in goji berry was developed by coupling of field amplified sample stacking (FASS) with an electroosmotic (EOF) pump driving water removal process. Due to the EOF pump and electrokinetic injection showing different influence on the concentration, the analytes injection condition should be systemically studied. Thereafter, the verification of the analytes injection conditions was achieved using response surface experimental design. Under the optimum conditions, 86-271 folds sensitivity enhancement upon normal capillary zone electrophoresis (CZE, 50 mbar x 5 s) were achieved for six flavonoids, and the detection limits ranged from 0.35 to 1.82 ng/mL; the LOQ ranged from 1.20 to 6.01 ng/mL. Eventually, the proposed method was applied to detect flavonoids in 30 goji berry samples from different habitats of China; and the results indicated that the flavonoids were rich in the eluent of 30-60% methanol, which provided a reference for extraction of goji berry flavonoids.
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