Journal
ELECTROPHORESIS
Volume 39, Issue 15, Pages 1957-1965Publisher
WILEY
DOI: 10.1002/elps.201800115
Keywords
Gas chromatography-mass spectrometry (GC-MS); Mentha rotundifolia; Microwave assisted extraction (MAE); Terpenoids
Funding
- Fundacion Ramon Areces [CIVP17A2843]
- Ministerio de Economia, Industria y Competitividad (MINEICO) of Spain [AGL2016-80475-R]
- Comunidad Autonoma of Madrid
- FEDER program [S2013/ABI-3028]
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A new microwave-assisted extraction (MAE) method using ethanol as solvent has been optimized by means of a Box-Behnken experimental design for the enhanced extraction of bioactive terpenoids from Mentha rotundifolia leaves; 100 degrees C, 5min, 1.125g dry sample: 10mL solvent and a single extraction cycle were selected as optimal conditions. Improved performance of MAE method in terms of extraction yield and/or reproducibility over conventional solid-liquid extraction and ultrasound assisted extraction was also previously assessed. A comprehensive characterization of MAE extracts was carried out by GC-MS. A total of 46 compounds, mostly terpenoids, were identified; piperitenone oxide and piperitenone were the major compounds determined. Several neophytadiene isomers were also detected for the first time in MAE extracts. Different procedures (solid-phase extraction and activated charcoal (AC) treatment) were also evaluated for clean-up of MAE extracts, with AC providing the highest enrichment in bioactive terpenoids. Finally, the MAE method here developed is shown as a green, fast, efficient and reproducible liquid extraction methodology to obtain M. rotundifolia bioactive extracts for further application, among others, as food preservatives.
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