Journal
ELECTROCHIMICA ACTA
Volume 268, Issue -, Pages 554-561Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.electacta.2018.02.094
Keywords
Electrochemical bioimaging; Mesenchymal stem cells; Cell analysis; Electrode array device; Osteogenesis
Categories
Funding
- Special Coordination Funds for Promoting Science and Technology
- Creation of Innovation Centers for Advanced Interdisciplinary Research Areas Program from the Japan Science and Technology Agency
- Asahi Glass Foundation
- [16H02280]
- [15H035420]
- [15H05415]
- Grants-in-Aid for Scientific Research [15H03542, 16H02280, 15H05415] Funding Source: KAKEN
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Mesenchymal stem cells (MSCs) have received great attention for tissue engineering. In the present study, the early stage of osteogenesis of MSC aggregates was monitored by electrochemicolor imaging using an electrode array device that can simultaneously detect multiple redox species. Electrochemicolor imaging was used to evaluate the cells' endogenous alkaline phosphatase (ALP) and respiratory activities. High ALP activity was observed in the aggregates after osteomedium (OM) treatment, with hydroxyapatite addition inducing early-stage osteogenesis in large aggregates. In small aggregates, OM treatment decreased the respiratory activity. To the best of our knowledge, this is the first report to use electrochemical imaging of ALP and respiratory activities to evaluate osteogenesis in MSC aggregates. (c) 2018 Elsevier Ltd. All rights reserved.
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