The capture proteasome assay (CAPA) to evaluate subtype-specific proteasome inhibitors

We recently developed a new assay to measure proteasome activity in vitro (CAPA for capture proteasome assay) [1] based on proteasome capture on an antibody -coated plate. When used with lysates originating from cells expressing either standard proteasome, immunoproteasome or intermediate proteasomes beta 5i or beta 1i-beta 5i, this assay allows the individual monitoring of the chymotrypsin-like, trypsin-like and caspase-like activities of the corresponding proteasome subtypes. The efficiency and specificity of four proteasome inhibitors were studied using the CAPA assay, demonstrating the potential of this assay for the development of subtype-specific proteasome inhibitors. (C) 2015 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license.