Journal
DYES AND PIGMENTS
Volume 149, Issue -, Pages 224-228Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.dyepig.2017.09.067
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Funding
- National Science Centre (Poland) [2015/17/B/ST5/03143]
- Polish State Committee for Scientific Research [DS/530-8457-D603-17]
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The article presents an approach for determination of low activity hydrolases down to the nanoscale amount. It consists in a specific design of fluorescent indicator using Excited-State Intramolecular Proton-Transfer (ESIPT) phenomenon and enhancement of its fluorescence on the metal surface (Metal Enhanced Fluorescence - MEF). The developed approach is tested with beta-glucosidase using novel ESIPT fluorescent indicator from the flavonol family.
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