Journal
DYES AND PIGMENTS
Volume 155, Issue -, Pages 171-178Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.dyepig.2018.03.057
Keywords
Malachite and leucomalachite green; CdTe QDs; Fluorescence resonance energy transfer; Inner filter effect; Dual-template molecularly imprinted polymer
Funding
- Foundation from the Science and Technology Planning Project of Fujian Province, China [2016Y0064]
- opening project of Fujian Provincial Engineering Technology Research Center of Marine Functional Food [C11172]
- Innovative Research Team of Jimei University, China [2010A007]
- Natural Science Foundation of Fujian Province of China [2015J01615, 2017J01633]
- National Undergraduate Training Programs for Innovation and Entrepreneurship [201610390042, 201710390022]
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Malachite green (MG) is an industrial dye, but has been once abused in aquaculture for disease treatment. Because MG and its metabolite, leucomalachite green (LMG), present great harm to human health, it is essential to rapidly and accurately detect MG and LMG residues in aquatic products. However, the simultaneous screening of MG and LMG is still a challenge to date. In this work, a dual-template molecularly imprinted polymer (MIP) coated CdTe QDs was developed and applied as a nano-probe to simultaneously detect MG and LMG in fish samples via normal and synchronous fluorescence quenching. The molar ratios of templates (MG and LMG) to monomer (APTES) and cross-linker (TEOS), and the amount of CdTe QDs in the probe preparation were optimized. A normal fluorescence emission at 600 nm could be linearly quenched by MG, while the synchronous fluorescence emission at the wavelength interval of 340 nm could be quenched by both LMG and MG. The detection limits of MG and LMG were 17 nmol L-1 and 28 nmol L-1 (3 sigma, n = 9), respectively. Based on the equations of Stem-Volmer, MG and LMG could be simultaneously detected without any conversion treatment. The recoveries of MG and LMG in fish samples ranged from 92% to 107% and from 91% to 108%, respectively. The results demonstrated that the as-prepared dual-template CdTe@MIP could be used as a fluorescent probe to rapidly and simultaneously detect MG and LMG in fish samples.
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