Journal
DYES AND PIGMENTS
Volume 159, Issue -, Pages 45-48Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.dyepig.2018.05.069
Keywords
Glutathione; Chromo-fluorogenic detection; Cu(II) complex; Displacement assay
Funding
- Spanish Government [MAT2015-64139-C4-1-R]
- Generalitat Valenciana [PROMETEOII/2014/047]
- Generalitat Valenciana
- Fundacao para a Ciencia e Tecnologia (Portugal)
- FCT
- FEDEReCOMPETEQREN-EU [UID/QUI/0686/2016]
- Infrastructure Project [022161]
- FEDER through COMPETE 2020
- FEDER through POCI
- FEDER through PORL
- FCT through PIDDAC
- [SFRH/BD/86408/2012]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/86408/2012] Funding Source: FCT
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The imidazole derivative 4-(4,5-diphenyl-1H-imidazol-2-yl)-N,N-dimethylaniline (probe 1) formed a highly coloured and non-emissive 1:1 stoichiometry complex with Cu(II) in water-acetonitrile 1:1 (v/v) solutions. Among all the amino acids (Lys, Val, Gln, Leu, His, Thr, Trp, Gly, Phe, Arg, Ile, Met, Ser, Ala, Pro, Tyr, Gly, Asn, Asp, Glu, Cys and Hcy) and tripeptides (GSH) tested, only GSH induced the bleaching of the 1 center dot Cu(II) solution together with a marked emission enhancement at 411 nm (excitation at 320 nm). These chromo-fluorogenic changes were ascribed to a selective GSH-induced demetallation of the 1 center dot Cu(II) complex that resulted in a recovery of the spectroscopic features of probe 1. In addition to the remarkable selectivity of 1-Cu(II) complex toward GSH a competitive limit of detection as low as 2 mu M was determined using fluorescence measurements.
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