4.3 Article

Detection of Zika, dengue and chikungunya viruses using single-reaction multiplex real-time RT-PCR

Journal

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
Volume 92, Issue 4, Pages 284-287

Publisher

ELSEVIER SCIENCE INC
DOI: 10.1016/j.diagmicrobio.2018.06.019

Keywords

Zika; Dengue; Chikungunya; PCR; Molecular; Multiplex

Funding

  1. Delegation Regionale a la Recherche Clinique des Hopitaux de Toulouse

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Zika (ZIKV), Dengue (DENV) and Chikungunya viruses (CHIKV) co-circulate in the same geographical areas during the same seasonal period through the same biting arthropods. Therefore a rapid sensitive and specific molecular assay for these viruses would be a considerable help in the disease management and the epidemiological survey. We developed a one-step multiplex real-time PCR for the simultaneous detection of these viruses. Intra and inter-reproducibilities varied from 0.41% to 3.29% and from 1.13% to 4.93% for each virus respectively. The specificity was 100%. Whole blood, plasma and urines were used for comparison with commercially available monoplex assays (RealStar (R) kits, Altona Diagnostics GmbH, Hamburg, Germany). The concordance was 96%, 92.9% and 95.7% for ZIKV, DENV and CHIKV respectively. No cross reaction and no PCR inhibition were observed for any of the clinical samples. This test can thus be used as a rapid molecular assay for ZIKV, DENVI-4 and CHIKV infections. (C) 2018 Published by Elsevier Inc.

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