Journal
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
Volume 91, Issue 2, Pages 118-122Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.diagmicrobio.2018.01.026
Keywords
16S rRNA methylases; Antibiotic; Susceptibility testing; Gentamicin; Amikacin; Tobramycin; Netilmicin; Kanamycin; Plazomicin
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Funding
- University of Fribourg, Switzerland
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The amikacin plus gentamicin-containing Super Aminoglycoside medium was developed for screening multipleaminoglycoside resistance in Gram-negative bacteria (Enterobacteriaceae, Pseudomonas aeruginosa, and Acinetobacter baumannii). It was evaluated using aminoglycoside-susceptible (n=12) and aminoglycoside-resistant (n=59) Gram-negative isolates, including 16S rRNA methylase producers (n=20). Its sensitivity and specificity of detection were, respectively, of 95% and 96% for detecting multiple aminoglycoside-resistant methylase producers. (C) 2018 Elsevier Inc. All rights reserved.
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