Journal
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
Volume 90, Issue 2, Pages 90-95Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.diagmicrobio.2017.10.005
Keywords
Respiratory syncytial virus; Detection; Subgroup; RT-RAA
Categories
Funding
- China Mega-Project for Infectious Disease [2016ZX10004-101]
- Beijing Municipal Science and Technology Commission project [D151100002115003]
- [2016TFC1202700]
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In this study, a rapid reverse-transcription recombinase aided amplification (RT-RAA) assay was developed to detect respiratory syncytial virus (RSV) subgroups A and B, respectively. The reaction was performed at 39 C in less than 30 min. The analytical sensitivities of RSVA and RSVB at 95% probability by probit regression analysis were 38copies per reaction and 35 copies per reaction, respectively, and no cross reactions with other related respiratory viruses were observed. The RT-RAA assay was further utilized to detect and subgroup 306 clinical specimens and the results showed that 79(25.82%, 79/306) samples were positive for RSV, of those 16(20.25%, 16/79) were identified as RSVA and 63(79.75%, 63/79) were RSVB, which is completely consistent with the results obtained by RSV RT-qPCR assay. In conclusion, the developed RAA assay will be of benefit as a faster, sensitive and specific alternative tool for detection of RSV. (C) 2017 Elsevier Inc. All rights reserved.
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