Journal
BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY
Volume 4, Issue 3, Pages 327-334Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bcab.2015.06.002
Keywords
Chlorogenate hydrolase; Chlorogenic acid; Yerba mate (Ilex puraguariensis); Aspergillus niger; Inducible enzymes
Categories
Funding
- National Institute of Yerba Mate (INYM, Argentina (PRASY)) [049/11]
- Argentina National Research Council (CONICET) [PIP 0662]
- National University of La Plata (UNLP) [11/X650]
- JSPS KAKENHI [23580135]
- Grants-in-Aid for Scientific Research [26450094, 23580135] Funding Source: KAKEN
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The potential of yerba mate (Ilex paraguariensis) to induce chlorogenate hydrolase (EC 3.1.1.42, CHase) activity in different fungal strains has been investigated. CHase was highly induced in mycelia of Aspergillus niger AKU 3302 grown in Czapek medium containing yerba mate extract as inducer. Different materials derived from yerba mate processing were examined as CHase activity inducers. All yerba mate samples produced CHase activity and a concentrated aqueous yerba mate extract was selected as the most convenient inducer. A response surface methodology was used to assess the simultaneous effect of inducer concentration and induction duration on enzyme production. The experimental design revealed that the optimum CHase production was achieved after long induction times (>= 25 h) and low inducer concentrations (01-0.2% wiv, dry basis). (C) 2015 Elsevier Ltd. All rights reserved
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