Journal
BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY
Volume 4, Issue 2, Pages 221-228Publisher
ELSEVIER
DOI: 10.1016/j.bcab.2014.12.004
Keywords
Screening; Culture medium design; Fermentation optimization; Waste waters
Categories
Funding
- Ministerio de Ciencia, Tecnologia e Innovacion Productiva de la Nacion Argentina
- Universidad Nacional de Quilmes
Ask authors/readers for more resources
Pectin degrading enzymes with polygalacturonase (PG) activity hydrolyze alpha'-(1,4) glycosidic bonds of polysaccharides present in higher plants. In the current study one hundred bacterial strains were screened for extracellular PG activity using an inductive culture medium. Optimization of fermentation conditions for Streptomyces halstedii ATCC 10897 was conducted using experimental designs. The maximum enzymatic activity obtained was 3.489 UfmL and 98.0% of viscosity reduction after 12 h of fermentation using soy peptone as unique source of carbon and nitrogen. PG from S. halstecth ATCC 10897 showed high thermal stability, an approximate molecular weight of 48 kDa and its optimum conditions for catalytic reaction were 50 degrees C and pH 12.0. This study reveals that alkaline PG is a useful enzyme for depectinization in alkaline pulping mill and papermaking waste waters. (C) 2015 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available