Studies on endosulfan degradation by local isolate Pseudomonas aeruginosa

A bacterium capable of tolerating high levels of endosulfan was isolated from soil of cotton field. The ability of the bacterium to degrade endosulfan was investigated by culturing it in minimal media and nutrient broth containing high levels of endosulfan. The 165 rRNA gene sequencing indicated the bacterium as Pseudomonas aeniginosa. Endosulfan degradation by the bacteria was found to occur more rapidly in media with supportive nutrients than the minimal media. P. aeruginosa was able to degrade 96% at endosulfan after 288 h under static conditions. The metabolites formed during endosullan degradation were analyzed by using UV visible spearophoLomeLry and thin layer chromatography. The complete mineralization of enclosulfan was found to occur after 28 days. The levels of clehydrogenase, arylsulfaLase, dehalogenase enzymes and CO2 were monitored to Lest the soil activity. The terminal products formed by bacteria during biodegradation were tested for phylo-LoxiciLy. This study will be useful for the development of bioremecliaLion strategies. (C) 2015 Elsevier Ltd. All rights reserved,