Journal
BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY
Volume 4, Issue 1, Pages 55-62Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bcab.2014.07.001
Keywords
Enzymatic hydrolysis; Egg white protein; Antioxidant activities
Categories
Funding
- Sao Paulo Research Foundation - FAPESP [2011/10429-9]
- National Council of Technological and Scientific Development - CNPq
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [11/10429-9] Funding Source: FAPESP
Ask authors/readers for more resources
Bioactive peptides can be defined as specific protein fractions with amino acid sequence that promotes a positive impact on several biologic activities. The hydrolysis of egg white protein might result in releasing or enhancing their biological activity. The aim of this study was to evaluate the effects of the enzymatic hydrolysis of egg white proteins on their antioxidant properties. The hydrolysates were prepared with protease from Aspergillus cuyzae LBA 01 (AO) produced under solid-state fermentation and two commercial proteases: protease from A. oryzae (CAD), and Bacillus lichenifortnis (CBL). The enzymatic hydrolysis with microbial proteases increased the antioxidant properties of egg white proteins. The egg white protein hydrolysates prepared with commercial protease CAD, presented higher antioxidant activity than the hydrolysates obtained with the other proteases. According CCRD analysis, the hydrolysates prepared with 3.0% of egg white proteins and 20.0 U of protease per mL of reaction mixture, presented high radical-scavenging effect, with an ORAC value of 119312 +/- 84.62 and MTH value of 19.05 +/- 0.81 Trolox EQ mu mol g(-1) In ORAC assay, the maximum antioxidant activities were observed in the hydrolysates presenting DH 50.0% obtained after 120 min of hydrolysis. In contrast, for DRPH assay the highest values of antioxidant activity were detected in the hytholysates obtained in the first 30 min, where the degree of hydrolysis was greater than 60.0%. (C) 2014 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available