Journal
DIABETES
Volume 67, Issue 7, Pages 1369-1379Publisher
AMER DIABETES ASSOC
DOI: 10.2337/db17-1124
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Funding
- National Institutes of Health (NIH)/National Center for Research Resources [T32-DK-063687, K23-RR-020038, 1R56-DK-088971]
- NIH Building Interdisciplinary Research Careers in Women's Health [2K12-HD-057022, K23-DK-107871]
- Doris Duke Foundation [2015212]
- Denver Research Institute pilot
- Eastern Colorado Geriatric Research, Education, and Clinical Center
- American Diabetes Association Clinical Research Grant [1-12-CT-64]
- Veterans Administration Merit Award [CVP BX002046]
- NIH/National Center for Advancing Translational Sciences [Colorado CTSA UL1-TR-001082]
- Magnet NIH [1S10-OD-018435]
- Eugene Armstrong Family Foundation
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Type 2 diabetes is associated with impaired exercise capacity. Alterations in both muscle perfusion and mitochondrial function can contribute to exercise impairment. We hypothesized that impaired muscle mitochondrial function in type 2 diabetes is mediated, in part, by decreased tissue oxygen delivery and would improve with oxygen supplementation. Ex vivo muscle mitochondrial content and respiration assessed from biopsy samples demonstrated expected differences in obese individuals with (n = 18) and without (n = 17) diabetes. Similarly, in vivo mitochondrial oxidative phosphorylation capacity measured in the gastrocnemius muscle via P-31-MRS indicated an impairment in the rate of ADP depletion with rest (27 +/- 6 s [diabetes], 21 +/- 7 s [control subjects]; P = 0.008) and oxidative phosphorylation (P = 0.046) in type 2 diabetes after isometric calf exercise compared with control subjects. Importantly, the in vivo impairment in oxidative capacity resolved with oxygen supplementation in adults with diabetes (ADP depletion rate 5.0 s faster, P = 0.012; oxidative phosphorylation 0.046 +/- 0.079 mmol/L/s faster, P = 0.027). Multiple in vivo mitochondrial measures related to HbA(1c). These data suggest that oxygen availability is rate limiting for in vivo mitochondrial oxidative exercise recovery measured with P-31-MRS in individuals with uncomplicated diabetes. Targeting muscle oxygenation could improve exercise function in type 2 diabetes.
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