Journal
DEVELOPMENTAL BIOLOGY
Volume 433, Issue 2, Pages 262-275Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ydbio.2017.10.008
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Funding
- Portuguese Foundation for Science and Technology (FCT) [SFRH/BD/51280/2010]
- central fund from CRTD
- central fund from MPI-CBG
- Fundação para a Ciência e a Tecnologia [SFRH/BD/51280/2010] Funding Source: FCT
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Axolotls can regenerate complex structures through recruitment and remodeling of cells within mature tissues. Accessing the underlying mechanisms at a molecular resolution is crucial to understand how injury triggers regeneration and how it proceeds. However, gene transformation in adult tissues can be challenging. Here we characterize the use of pseudotyped baculovirus (BV) as an effective gene transfer method both for cells within mature limb tissue and within the blastema. These cells remain competent to participate in regeneration after transduction. We further characterize the effectiveness of BV for gene overexpression studies by overexpressing Shh in the blastema, which yields a high penetrance of classic polydactyly phenotypes. Overall, our work establishes BV as a powerful tool to access gene function in axolotl limb regeneration.
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