Journal
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
Volume 87, Issue -, Pages 64-74Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2018.05.022
Keywords
T lymphocyte; Immunosenescence; Inflammaging; Canine; CD45RA
Categories
Funding
- UC Davis Center for Companion Animal Health
- NIH-NCI [1R01CA195904-01, P30CA093373]
- NIH-NCIU01 [CA224166-01]
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While dogs are increasingly being utilized as large-animal models of disease, important features of age-related immunosenescence in the dog have yet to be evaluated due to the lack of defined naive vs. memory T lymphocyte phenotypes. We therefore performed multi-color flow cytometry on peripheral blood mononuclear cells from young and aged beagles, and determined the differential cytokine production by proposed memory subsets. CD4 + and CD8 + T lymphocytes in aged dogs displayed increased cytokine production, and decreased proliferative capacity. Antibodies targeting CD45RA and CD62L, but less so CD28 or CD44, defined canine cells that consistently exhibited properties of naive-, central memory-, effector memory-, and terminal effector-like CD4 + and CD8 + T lymphocyte subsets. Older dogs demonstrated decreased frequencies of naive-like CD4 + and CD8+ T lymphocytes, and an increased frequency of terminal effector-like CD8 + T lymphocytes. Overall findings revealed that aged dogs displayed features of immunosenescence similar to those reported in other species.
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