Journal
PHARMACOGNOSY RESEARCH
Volume 7, Issue 4, Pages 309-313Publisher
MEDKNOW PUBLICATIONS & MEDIA PVT LTD
DOI: 10.4103/0974-8490.158442
Keywords
Alpha-amylase inhibitor; hispidulin-7-O-(4-O-acetyl-rutinoside); hispidulin-7-O-rutinoside; linarin; Zhumeria majdae
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Funding
- Tehran University of Medical Sciences and Health Services grant [21583]
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Background: Zhumeria majdae (Lamiaceae) is an endemic species growing in the South parts of Iran especially Hormozgan province. The plant is so-called Mohrekhosh locally and widely used for medicinal purposes including stomachache and dysmenorrhea. Objective: In order to separation and identification of the main flavonoid glycosides of the plant (aerial parts including leaves, stems, flowers, and fruits were used) and evaluation of its alpha-amylase inhibitory (AAI) activity, methanolic extract was prepared and fractionated to botanolic portion. Materials and Methods: Isolation of the main compounds of the butanol extract of the plant have been performed using different column chromatography methods such as high-performance liquid chromatography (C-18 column) and Sephadex LH-20 as well. The isolated compounds were identified by Hydrogen-1 nuclear magnetic resonance and Carbon-13 nuclear magnetic resonance spectra and comparison with those reported in previous literature. Moreover, inhibitory activity of the butanolic extract of the plant against alpha-amylase enzyme was examined in different concentrations (15-30 mg/mL), where acarbose used as a positive control. Results: Three flavonoid glycosides: Linarin (1), hispidulin-7-O-(4-O-acetyl-rutinoside) (2), hispidulin-7-O-rutinoside (3) were successfully identified in the extract. The activity of alpha amylase enzyme was dose-dependently suppressed by the butanol extract. The extract exhibited the highest inhibition at 30 mg/mL toward enzyme (77.9 +/- 2.1%), while acarbose inhibited the enzyme at 20 mg/mL by 73.9 +/- 1.9%. The inhibitory concentrations of 50% for the extract and acarbose were calculated at 24.5 +/- 2.1 and 6.6 +/- 3.1 mg/mL, respectively. Conclusion: Z. majdae contains glycosylated flavones and could be a good candidate for anti-diabetic evaluations in animal and clinical trials due to possessing AAI activity.
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