Effect of fluoride mouthrinse on adhesion to bovine root dentin

The purpose of this study was to evaluate the effect of fluoride mouthrinses on dentin bonding performance of a two-step self-etch adhesive; Clearfil SE Bond. Bovine root dentin surface was treated with either 450, 900, or 9,000 ppm F solutions for 30 s (immediate), and continually treated for one month (one month) before the bonding procedures. Microtensile bond strength (mu TBS) test and scanning electron microscopic (SEM) observation of the acid-base resistance zone (ABRZ) were performed. Chemical state of fluorine on dentin surface was analyzed by X-ray absorption fine structure (XAFS). The 450 and 900 ppm F fluoride mouthrinses did not influence the mu TBS to dentin, while the 9,000 ppm F fluoride solution adversely affected the mu TBSs. The fluoride application to dentin significantly enhanced acid resistance at the adhesive/dentin interface including the mu TBSs. The XAFS analysis indicated different concentrations of fluoride might create different chemical compounds on the dentin surface, influencing the mu TBS results.