Journal
CURRENT PHARMACEUTICAL ANALYSIS
Volume 14, Issue 5, Pages 443-449Publisher
BENTHAM SCIENCE PUBL LTD
DOI: 10.2174/1573412913666170525163234
Keywords
Chondroitin sulfate; HILIC-MS/MS; disaccharides; MRM; hyaluronic acid; glycosaminoglycan
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Funding
- National Natural Science Foundation of China [31301431, 31501439]
- Public Science and Technology Research Funds Projects of Ocean [201505029]
- Dalian High-level Talents Innovation and Entrepreneurship Project [2016RQ061]
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Background: Acidic polysaccharides usually possess favorable bioactivities, and some of them are applied in medicines and dietary supplements together. However, it is a challenge to quantify these polysaccharides simultaneously. Methods: A method to Quantify Hyaluronic Acid (HA), Chondroitin Sulfate (CS) and a non-glycosaminoglycan acidic polysaccharide named AGSP was established by determining the contents of their disaccharides produced from the acid hydrolysis using hydrophilic interaction chromatography-tandem triple quadrupole mass spectrometry in the positive mode. Results: This method had excellent linearities in a concentration range of 0.02-2.00 mg/mL for these standard acidic polysaccharides, the recoveries were all 95%-112%, and relative standard deviations (RSDs) were less than 5%. Limit of Detection (LOD) and Limit of Quantification (LOQ) ranged from 0.1 mu g/mL to 4.1 mu g/mL. It could be inferred that the demonstrated method was feasible to quantitatively detect uronic acid-containing polysaccharides. Conclusion: This method could be a useful tool for quality control of medicines and dietary supplements containing these acidic polysaccharides.
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