Journal
PLANT SIGNALING & BEHAVIOR
Volume 10, Issue 5, Pages -Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/15592324.2015.1017177
Keywords
abscisic acid (ABA); artificial microRNA (amiRNA); aquaporins (AQPs); bundle sheath; leaf hydraulic conductivity (K-leaf); plasma membrane intrinsic proteins (PIPs)
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The role of molecular mechanisms in the regulation of leaf hydraulics (K-leaf) is still not well understood. We hypothesized that aquaporins (AQPs) in the bundle sheath may regulate K-leaf. To examine this hypothesis, AQP genes were constitutively silenced using artificial microRNAs and recovery was achieved by targeting the expression of the tobacco AQP (NtAQP1) to bundle-sheath cells in the silenced plants. Constitutively silenced PIP1 plants exhibited decreased PIP1 transcript levels and decreased K-leaf. However, once the plants were recovered with NtAQP1, their K-leaf values were almost the same as those of WT plants. We also demonstrate the important role of ABA, acting via AQP, in that recovery and K-leaf regulation. These results support our previously raised hypothesis concerning the role of bundle-sheath AQPs in the regulation of leaf hydraulics.
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