Journal
AQUACULTURE REPORTS
Volume 2, Issue -, Pages 106-111Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aqrep.2015.08.009
Keywords
Crustin; Shrimp; Penaeus monodon; Vibrio harveyi; Anti-bacterial activity
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Funding
- Indian Council of Agricultural Research (ICAR) through the National fund for basic, strategic and frontier application research in agriculture (NFBSFARA) project on 'Defense genes of shrimp'
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Crustaceans have a powerful non-specific immune mechanism that responds to pathogen invasion and together with cellular responses, generates powerful humoral factors such as antimicrobial peptides. Crustins are a diverse class of antimicrobial peptides that are expressed by the circulating haemocytes of crustaceans. Several isoforms of this molecule are reported and in this study, one isoform from the black tiger shrimp, Penaeus monodon was cloned and expressed in Escherichia coli SG 13009. The purified recombinant crustin peptide had a molecular weight of 22 kDa and exhibited potent anti-bacterial activity in vitro against several Gram positive and Gram negative bacteria that included pathogens of aquatic animals and humans. The recombinant crustin showed a minimal inhibitory concentration of 0.5 mu g ml(-1) against the vibrio pathogens of shrimp, which suggests its promise for application in aquaculture. (C) 2015 The Authors. Published by Elsevier B.V.
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