Journal
FOOD RESEARCH INTERNATIONAL
Volume 73, Issue -, Pages 124-129Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.foodres.2014.08.038
Keywords
Bluefin leatherjacket (Navodon septentrionalis); Skin; Protein hydrolysate; Peptide; Antioxidant activity
Categories
Funding
- National Natural Science Foundation of China (NSFC) [31001109, 31201452]
- International S&T Cooperation Program of China [2012DFA30600]
- Special Program for the S&T Plan of Zhejiang Province [2011C02003]
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Skin protein from a bluefin leatherjacket (Navodon septentrionalis) processing by-product was hydrolyzed by trypsin, flavourzyme, neutrase, papain, alcalase, and pepsin, and protein hydrolysate (BSH) prepared using alcalase showed the highest DPPH center dot, HO center dot, and O-2(-) center dot scavenging activities among all hydrolysates. Using ultrafiltration and consecutive chromatography, three novel peptides with strong antioxidant properties were purified from BSH, and their sequences were determined as Gly-Ser-Gly-Gly-Leu (GSGGL, BSP-A), Gly-Pro-Gly-Gly-Phe-Ile (GPGGFI, BSP-B), and Phe-Ile-Gly-Pro (FIGP, BSP-C) with molecular weights of 389.41, 546.63, and 432.52 Da, respectively. BSP-C exhibited the highest scavenging activities on DPPH center dot (EC50 0.118 mg/ml), HO center dot (EC50 0.073 mg/ml), and O-2(-) center dot (EC(50)0.311 mg/ml) among the three peptides. In addition, ESP-C could effectively inhibit autooxidation in a linoleic add model system. The antioxidant activities of BSP-A, BSP-B, and BSP-C might be due to the small molecular sizes and the hydrophobic and/or aromatic amino acid residues in their amino acid sequences. The present results suggested that peptides purified from the skin protein hydrolysate of bluefin leatherjacket were excellent antioxidants and could be effectively used as food ingredients and additives, and pharmaceuticals. (C) 2014 Elsevier Ltd. All rights reserved.
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