Journal
INTERNATIONAL JOURNAL OF TECHNOLOGY
Volume 6, Issue 1, Pages 3-10Publisher
UNIV INDONESIA
DOI: 10.14716/ijtech.v6i1.784
Keywords
Acid pretreatment; Bioethanol; Enzymatic hydrolysis; Rice straw; Second generation biofuel production
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Funding
- Exploratory Research Grant Scheme (ERGS) through the Ministry of Higher Education of Malaysia (MOHE) [ERGS/1/2013/TK07/UNITEN/01/01]
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Clean, safe and sustainable energy sources must be found to minimize all side-effects of fossil fuel consumption. Second generation bioethanol possesses a great potential as an alternative energy source especially in the transportation sector. In this study, rice straw was selected to be studied as a conversion of potential lignocellulosic biomass into bioethanol. Firstly, rice straw was processed with mechanical pretreatment using a home blender, followed by acid pretreatment using 2.0 M sulphuric acid (H2SO4) at 90 degrees C for 60 minutes. The glucose yield was found to be 9.71 g/L. Then, rice straw pretreated with acid was hydrolyzed using 24 mg of cellulase from Tichoderma Ressei ATCC 26921 over a 72-hour duration, which yielded a total glucose count of 11.466 g/L. After fermentation with Saccharomyces cerevisiae, it was found that by combining enzymatic hydrolysis with acid pretreatment yielded a higher ethanol content after fermentation (0.1503% or 52.75% of theoretical value) compared to acidic pretreatment alone (0.013% or 11.26% of theoretical value).
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