Journal
FOOD HYDROCOLLOIDS
Volume 43, Issue -, Pages 228-235Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodhyd.2014.05.022
Keywords
Glycosylated bovine serum albumin; Epigallocatechin-3-gallate; Binding
Categories
Funding
- United States Department of Agriculture National Research Initiative [2009-35603-05071]
- National 125 Program of China [2011BAD23B04]
- National Natural Science Foundation for Young Scholars of China [31000815]
- PAPD
Ask authors/readers for more resources
Polyphenols normally have strong binding affinity with proteins which often leads to precipitation. Glycosylation of protein via Maillard reaction in mild conditions may inhibit the precipitation and thus is beneficial for the development of controlled-releasable bioactive materials. In this paper, we employed fluorescence spectroscopy, small-angle x-ray scattering (SAXS), and dynamic light scattering to study the influence of the protein glycosylation on the epigallocatechin-3-gallate (EGCG) induced interaction and aggregation behaviors of bovine serum albumin (BSA). Two dextran molecules with molecular weights of 10 kDa (DT10) and 20 kDa (DT20) were used for protein glycosylation. The protein-dextran conjugates were found to have a core-shell structure, with BSA core radius of 4.2 nm and the dextran surrounding layer thickness of 2.9 nm and 2.4 nm, respectively. In the presence of EGCG, large-scale aggregation was ultimately suppressed in the mixture of BSA-dextran conjugate and EGCG, in contrast to the mixture of BSA and EGCG when the dextran conjugate layer was introduced. The hydrophilic layer of dextran inhibited the interaction between BSA and EGCG which was dominated by hydrophobic interaction and/or hydrogen bonding, which has also been verified by the much lower binding affinity of EGCG with BSA-dextran conjugates than with the native protein (i.e., BSA). This work clearly shows that the glycosylation does not have any positive effect of aggregation of BSA when change the molar ratio of EGCG to protein in 100-200 times, which will be promising for polyphenol based nano-carriers development. (C) 2014 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available